| Title: | The relationship between polyunsaturated fatty acids and inflammation: evidence from cohort and Mendelian randomization analyses |
| Journal: | International Journal of Epidemiology |
| Published: | 11 Jun 2025 |
| Pubmed: | https://pubmed.ncbi.nlm.nih.gov/40550517/ |
| DOI: | https://doi.org/10.1093/ije/dyaf065 |
Publication 16264
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Abstract
BACKGROUND: Omega-3 (n-3) and omega-6 (n-6) polyunsaturated fatty acids (PUFAs) are thought to have anti- and pro-inflammatory roles, respectively, and influence the risk of various chronic diseases. However, it is unclear whether these associations are causal.</p>
METHODS: We examined the associations of dietary polyunsaturated FAs with biomarkers of systemic inflammation: C-reactive protein (CRP), glycoprotein acetyls (GlycA), and interleukin 6 (IL-6) in two cohort datasets-Avon Longitudinal Study of Parents and Children (N = 2802) and UK Biobank (N = 12 401)-by using multivariable analyses. We investigated causality by using two-sample Mendelian randomization (MR). In addition to the inverse-variance weighted (IVW) method, we used sensitivity analyses to strengthen the causal inference. We conducted multivariable MR (MVMR) to investigate the causal effects of n-3 and n-6 on inflammation, accounting for the low-density lipoprotein (LDL) cholesterol, triglycerides, monounsaturated FAs, and saturated FAs.</p>
RESULTS: Cohort analyses show a positive association between the n-6:n-3 ratio and each biomarker. Total n-3 and n-6 PUFAs were associated with higher GlycA levels [mean difference = 0.33; 95% confidence interval (CI) = 0.29, 0.36, and 0.52; 95% CI = 0.48, 0.55, respectively]. The MR results suggest that total n-3 FAs cause higher circulating CRP (IVW = 0.09; 95% CI = 0.03, 0.16) and GlycA levels (0.12; 95% CI = 0.04, 0.21). The positive association between n-3 FAs and GlycA remained in the MVMR analysis after accounting for LDL cholesterol, triglycerides, monounsaturated FAs, and saturated FAs.</p>
CONCLUSION: We find no convincing evidence of a simple pro- and anti-inflammatory dichotomy regarding the function of n-6 and n-3 PUFAs. Further research is needed to better understand the mechanisms underlying the effects of PUFAs on specific immune biomarkers.</p>
17 Keywords
- Adult
- Biomarkers
- C-Reactive Protein
- Cholesterol, LDL
- Cohort Studies
- Diet
- Fatty Acids, Omega-3
- Fatty Acids, Omega-6
- Female
- Humans
- Inflammation
- Interleukin-6
- Longitudinal Studies
- Male
- Mendelian Randomization Analysis
- Middle Aged
- United Kingdom
5 Authors
- Daisy C P Crick
- Sarah L Halligan
- George Davey Smith
- Golam M Khandaker
- Hannah J Jones
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