| Title: | Probing the aggregated effects of purifying selection per individual on 1,380 medical phenotypes in the UK Biobank |
| Journal: | PLOS Genetics |
| Published: | 25 Jan 2021 |
| Pubmed: | https://pubmed.ncbi.nlm.nih.gov/33493176/ |
| DOI: | https://doi.org/10.1371/journal.pgen.1009337 |
| URL: | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1009337&type=printable |
| Citations: | 2 (2 in last 2 years) as of 8 Aug 2024 |
Publication 5610
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Abstract
Understanding the relationship between natural selection and phenotypic variation has been a long-standing challenge in human population genetics. With the emergence of biobank-scale datasets, along with new statistical metrics to approximate strength of purifying selection at the variant level, it is now possible to correlate a proxy of individual relative fitness with a range of medical phenotypes. We calculated a per-individual deleterious load score by summing the total number of derived alleles per individual after incorporating a weight that approximates strength of purifying selection. We assessed four methods for the weight, including GERP, phyloP, CADD, and fitcons. By quantitatively tracking each of these scores with the site frequency spectrum, we identified phyloP as the most appropriate weight. The phyloP-weighted load score was then calculated across 15,129,142 variants in 335,161 individuals from the UK Biobank and tested for association on 1,380 medical phenotypes. After accounting for multiple test correction, we observed a strong association of the load score amongst coding sites only on 27 traits including body mass, adiposity and metabolic rate. We further observed that the association signals were driven by common variants (derived allele frequency > 5%) with high phyloP score (phyloP > 2). Finally, through permutation analyses, we showed that the load score amongst coding sites had an excess of nominally significant associations on many medical phenotypes. These results suggest a broad impact of deleterious load on medical phenotypes and highlight the deleterious load score as a tool to disentangle the complex relationship between natural selection and medical phenotypes.4 Authors
- Ha My T. Vy
- Daniel M. Jordan
- Daniel J. Balick
- Ron Do
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