| Title: | Discovery of target genes and pathways at GWAS loci by pooled single-cell CRISPR screens |
| Journal: | Science |
| Published: | 19 May 2023 |
| Pubmed: | https://pubmed.ncbi.nlm.nih.gov/37141313/ |
| DOI: | https://doi.org/10.1126/science.adh7699 |
| URL: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10518238 |
Publication 11465
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Abstract
Most variants associated with complex traits and diseases identified by genome-wide association studies (GWAS) map to noncoding regions of the genome with unknown effects. Using ancestrally diverse, biobank-scale GWAS data, massively parallel CRISPR screens, and single-cell transcriptomic and proteomic sequencing, we discovered 124 cis-target genes of 91 noncoding blood trait GWAS loci. Using precise variant insertion through base editing, we connected specific variants with gene expression changes. We also identified trans-effect networks of noncoding loci when cis target genes encoded transcription factors or microRNAs. Networks were themselves enriched for GWAS variants and demonstrated polygenic contributions to complex traits. This platform enables massively parallel characterization of the target genes and mechanisms of human noncoding variants in both cis and trans.</p>
16 Authors
- John A Morris
- Christina Caragine
- Zharko Daniloski
- Júlia Domingo
- Timothy Barry
- Lu Lu
- Kyrie Davis
- Marcello Ziosi
- Dafni A Glinos
- Stephanie Hao
- Eleni P Mimitou
- Peter Smibert
- Kathryn Roeder
- Eugene Katsevich
- Tuuli Lappalainen
- Neville E Sanjana
1 Application
| Application ID | Title |
|---|---|
| 47976 | Genetic and environmental interactions in genetics of human traits: Insights from regulatory variation |
Enabling scientific discoveries that improve human health